ab104834 (1/200) staining PRMT6 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview
All lanes : Anti-PRMT6 antibody (ab104834) at 1 µg/mlLane 1 : A498 (Human Kidney Carcinoma) Whole Cell LysateLane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.