Anti-Profilin 1 antibody [EPR6304]

• Supplier: Abcam
• Catalog: ab124904
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6304
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide from the C terminal region of Human Profilin 1
• Description: Rabbit Monoclonal
• Gene: PFN1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab124904 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124904, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Profilin 1 antibody [EPR6304] (ab124904) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : JAR cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab124904, at 1/500 dilution, staining Profilin 1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

ab124904, at 1/250 dilution, staining Profilin 1 in HeLa cells by Immunofluorescence.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD