Anti-Prolactin Receptor antibody [T6]
| Name | Anti-Prolactin Receptor antibody [T6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2773 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | T6 |
| Applications | ICC/IF IHC-F WB IP FC B/N ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Sheep, Human |
| Antigen | Other Immunogen Type corresponding to Rat Prolactin Receptor |
| Description | Mouse Monoclonal |
| Gene | PRLR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in H-4-II-E cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in SW480 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized rat pituitary gland tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
Prolactin receptor expression in human hematopoietic tissues analyzed by flow - Prolactin receptor expression in human hematopoietic tissues analyzed by flow
Dardenne M, de Moraes Mdo C, Kelly PA, Gagnerault MC. Endocrinology. 1994 May;134(5):2108-14.
JAK2 activation and cell proliferation induced by antibody-mediated prolactin - JAK2 activation and cell proliferation induced by antibody-mediated prolactin
Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL. Endocrinology. 1994 Oct;135(4):1299-306.
Expression of prolactin receptors in murine lymphoid cells in normal and - Expression of prolactin receptors in murine lymphoid cells in normal and
Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M. J Immunol. 1993 Jun 15;150(12):5673-81.
Identification and functional activity of prolactin receptors in thymic - Identification and functional activity of prolactin receptors in thymic
Dardenne M, Kelly PA, Bach JF, Savino W. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9700-4.
Mutational analysis of the ligand-binding domain of the prolactin receptor. - Mutational analysis of the ligand-binding domain of the prolactin receptor.
Rozakis-Adcock M, Kelly PA. J Biol Chem. 1991 Sep 5;266(25):16472-7.
Characterization and applications of monoclonal antibodies to the prolactin - Characterization and applications of monoclonal antibodies to the prolactin
Okamura H, Zachwieja J, Raguet S, Kelly PA. Endocrinology. 1989 May;124(5):2499-508.