All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/mlLane 1 : Lung (Mouse) Whole Cell Lysate - normal tissue (ab29297)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Kidney (Mouse) Tissue LysateLane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 5 : EL4 (Mouse lymphoma cell line) Whole Cell Lysate (ab7183)Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : Small Intestine (Rat) Tissue Lysate Lane 4 : Kidney (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.