Anti-Prostaglandin dehydrogenase 1 antibody (ab96732) at 1/1000 dilution + Raji whole cell lysate at 30 µg
ab96732, at 1/500 dilution, staining prostagladin dehydrogenase 1 in Human MDA-MB-468 xenograft by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab96732 staining Prostaglandin dehydrogenase 1 in MCF7 cells treated with diclofenac sodium salt (ab120621), by ICC/IF. Increase in Prostaglandin dehydrogenase 1 expression correlates with increased concentration of diclofenac sodium salt as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ab120621 (diclofenac sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96732 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
ab96732, at 1/200 dilution, staining prostagladin dehydrogenase 1 by immunofluorescence analysis of paraformaldehyde-fixed HeLa cells.