All lanes : Anti-Prostaglandin dehydrogenase 1 antibody [EPR14332-19] (ab187161) at 1/1000 dilutionLane 1 : SW480 lysateLane 2 : LoVo lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Prostaglandin dehydrogenase 1 antibody [EPR14332-19] (ab187161) at 1/1000 dilutionLane 1 : Mouse small intestine lysateLane 2 : Rat small intestine lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of SW480 cells labeling Prostaglandin dehydrogenase 1 with ab187161 at 1/250 and DAPI staining (blue). An anti rabbit IgG (Alexa Fluor®555) was used as the secondary.