Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Proteasome 19S S5A with ab140683 at 1/5000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-Proteasome 19S S5A antibody (ab140683) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of Proteasome 19S S5A in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140683 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.