PSMD4

Gene Symbol PSMD4
Entrez Gene 5710
Alt Symbol AF, AF-1, ASF, MCB1, Rpn10, S5A, pUB-R5
Species Human
Gene Type protein-coding
Description proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Other Description 26S proteasome non-ATPase regulatory subunit 4|26S proteasome regulatory subunit S5A|S5a/antisecretory factor protein|angiocidin|antisecretory factor 1|multiubiquitin chain-binding protein
Swissprots Q9NS92 Q5VWC5 D3DV16 P55036
Accessions EAW53455 EAW53456 EAW53457 EAW53458 P55036 AB033605 BAA97581 BC002365 AAH02365 BC072008 AAH72008 JF432522 ADZ15739 U24704 AAB54057 U72664 AAB68598 XM_005245354 XP_005245411 XM_006711464 XP_006711527 NM_002810 NP_002801 NM_153822
Function Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Top Pathways Proteasome, Epstein-Barr virus infection

S5a/PSMD4 (D20B2) Rabbit mAb - 12441 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D20B2
Western blot analysis of extracts from various cell lines using S5a/PSMD4 (D20B2) Rabbit mAb.

S5a/PSMD4 (D17E4) Rabbit mAb - 3336 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D17E4
Western blot analysis of extracts from various cell lines using S5a/PSMD4 (D17E4) Rabbit mAb.

S5a/PSMD4 Antibody - 3846 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using S5a/PSMD4 Antibody.

pUb-R2 (L-15) - sc-13726 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

pUb-R2 (V-16) - sc-13725 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

pUb-R5 (AH1.1) - sc-53425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pUb-R5 (AH1.1): sc-53425. Western blot analysis of pUb-R5 expression in HeLa (A) and SK-MEL-28 (B) whole cell lysates and HeLa (C) and CCRF-CEM (D) nuclear extracts.

pUb-R5 (E-2) - sc-393546 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pUb-R5 (E-2): sc-393546. Western blot analysis of pUb-R5 expression in HeLa (A) and SK-MEL-28 (B) whole cell lysates and HeLa (C) and CCRF-CEM (D) nuclear extracts.

pUb-R5 (F-6) - sc-398033 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pUb-R5/R2 (F-6): sc-398033. Western blot analysis of pUb-R5/R2 expression in HeLa (A) and SK-MEL-28 (B) whole cell lysates.

pUb-R5 (C-20) - sc-13733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
pUb-R5 (C-20): sc-13733. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts and cytoplasmic staining of Leydig cells.

pUb-R5 (H-300) - sc-368048 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Proteasome 19S S5A antibody - ab140689 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Proteasome 19S S5A with ab140689 at 1/5000 (0.2µg/ml). Detection: DAB.

Anti-Proteasome 19S S5A antibody - ab140683 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Proteasome 19S S5A with ab140683 at 1/5000 (0.2µg/ml). Detection: DAB.

Anti-Proteasome 19S S5A antibody - ab154935 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Proteasome 19S S5A antibody (ab154935) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-Proteasome 19S S5A antibody - ab27916 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Proteasome 19S S5A antibody (ab27916) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + Raji cell lysateSecondaryHRP conjugated anti Rabbit IgG at 1/50000 dilution

Anti-Proteasome 19S S5A antibody - ab83344 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Proteasome 19S S5A antibody (ab83344) at 1 µg/ml + HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %SecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Proteasome 19S S5A antibody - ab28776 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Proteasome 19S S5A antibody (ab28776) at 0.3 µg/ml + Raji cell lysateSecondaryRabbit Anti-PSMD4 Antibody at 1/50000 dilution

Anti-Proteasome 19S S5A antibody - ab3318 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Proteasome 19S S5A antibody - ab20023 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Proteasome 19S S5A antibody [18] - ab106202 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 18
Anti-Proteasome 19S S5A antibody [18] (ab106202) at 1/1000 dilution + Jurkat whole cell lysate

Anti-Proteasome 19S S5A antibody [21] - ab106186 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 21
Anti-Proteasome 19S S5A antibody [21] (ab106186) at 1/1000 dilution + Jurkat whole cell lysate

Anti-Proteasome 19S S5A antibody [AH1.1] - ab20239 from Abcam

Prices $401.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone AH1.1
Proteasome 19S S5A was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Proteasome 19S S5A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab20239.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 41kDa; Proteasome 19S S5A

Anti-Proteasome 19S S5A antibody [EPR8936] - ab137109 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8936
All lanes : Anti-Proteasome 19S S5A antibody [EPR8936] (ab137109) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : HepG2 cell lysateLane 4 : C6 cell lysateLane 5 : Raw264.7 cell lysateLane 6 : PC-12 cell lysateLane 7 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labelled Goat anti-Rabbit at 1/2000 dilution

S5a/Angiocidin Antibody - AF8036 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, AtT‑20 mouse pituitary tumor cell line, IMR‑32 human neuroblastoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8036) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S5a/Angiocidin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S5a/Angiocidin Antibody - NBP2-37888 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative Control. Lane 3: Overexpression lysate

Proteasome 19S S5A Antibody - NBP1-90821 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: Proteasome 19S S5A Antibody [NBP1-90821] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
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