Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231]
| Name | Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] |
|---|---|
| Supplier | Abcam |
| Catalog | ab22674 |
| Prices | $395.00 |
| Sizes | 50 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | MCP231 |
| Applications | FC ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Rat, Rabbit, Human, Yeast, Potato, Broad species |
| Antigen | Full length protein (dinitrophenylated proteasomes) |
| Description | Mouse Monoclonal |
| Gene | PSMA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
 used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/16697_Proteasome-20S-alpha-123567-Primary-antibodies-ab22674-2.jpg)
Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Insulin/IGF-1-mediated longevity is marked by reduced protein metabolism. - Insulin/IGF-1-mediated longevity is marked by reduced protein metabolism.
Stout GJ, Stigter EC, Essers PB, Mulder KW, Kolkman A, Snijders DS, van den Broek NJ, Betist MC, Korswagen HC, Macinnes AW, Brenkman AB. Mol Syst Biol. 2013 Jul 2;9:679.
RPN-6 determines C. elegans longevity under proteotoxic stress conditions. - RPN-6 determines C. elegans longevity under proteotoxic stress conditions.
Vilchez D, Morantte I, Liu Z, Douglas PM, Merkwirth C, Rodrigues AP, Manning G, Dillin A. Nature. 2012 Sep 13;489(7415):263-8.
Effects of beta-hydroxy-beta-methylbutyrate treatment in different types of - Effects of beta-hydroxy-beta-methylbutyrate treatment in different types of
Kovarik M, Muthny T, Sispera L, Holecek M. J Physiol Biochem. 2010 Dec;66(4):311-9.
Human proteasomes analysed with monoclonal antibodies. - Human proteasomes analysed with monoclonal antibodies.
Hendil KB, Kristensen P, Uerkvitz W. Biochem J. 1995 Jan 1;305 ( Pt 1):245-52.