All lanes : Anti-Proteasome 26S S10 antibody [EPR14455] (ab182576) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : K562 cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Proteasome 26S S10 using ab182576 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling Proteasome 26S S10 using ab182576 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.
Immunofluorescence analysis of 293 cells labeling Proteasome 26S S10 using ab182576 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Flow cytometry analysis of K562 cells labeling Proteasome 26S S10 using ab182576 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.