Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling Proteasome 26S S10 with ab188315 at 1/110 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
All lanes : Anti-Proteasome 26S S10 antibody [EPR14456(2)] (ab188315) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Proteasome 26S S10 antibody [EPR14456(2)] (ab188315) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Proteasome 26S S10 antibody [EPR14456(2)] (ab188315) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : Hela cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution