Anti-Proteasome subunit beta type 4 antibody
| Name | Anti-Proteasome subunit beta type 4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55628 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | IHC-P ICC/IF ICC/IF WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein corresponding to Human Proteasome subunit beta type 4 aa 1-265 |
| Description | Mouse Monoclonal |
| Gene | PSMB4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Proteasome subunit beta type 4 antibody (ab55628) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
Western blot against tagged recombinant protein immunogen using ab55628 Proteasome subunit beta type 4 antibody at 1ug/ml.
![Overlay histogram showing HeLa cells stained with ab55628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55628, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17014_Proteasome-subunit-beta-type-4-Primary-antibodies-ab55628-1.jpg)
Overlay histogram showing HeLa cells stained with ab55628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55628, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Identification of survival genes in human glioblastoma cells by small interfering - Identification of survival genes in human glioblastoma cells by small interfering
Thaker NG, Zhang F, McDonald PR, Shun TY, Lewen MD, Pollack IF, Lazo JS. Mol Pharmacol. 2009 Dec;76(6):1246-55.
Identification of a gastrin response element in the vesicular monoamine - Identification of a gastrin response element in the vesicular monoamine
Catlow K, Ashurst HL, Varro A, Dimaline R. J Biol Chem. 2007 Jun 8;282(23):17069-77. Epub 2007 Apr 17.