All lanes : Anti-Protein Kinase D2 antibody [3A1] (ab118987) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Protein Kinase D2 cDNALysates/proteins at 5 µg per lane.
ab118987 at 1/100 dilution staining Protein Kinase D2 in HEK293T cells transfected with either pCMV6-ENTRY Protein Kinase D2 overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
ab119 at 1/100 dilution staining Protein Kinase D2 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).