PRKD2

Gene Symbol PRKD2
Entrez Gene 25865
Alt Symbol HSPC187, PKD2, nPKC-D2
Species Human
Gene Type protein-coding
Description protein kinase D2
Other Description serine/threonine-protein kinase D2
Swissprots Q9P0T6 Q9Y3X8 Q8NCK8 Q9BZL6 Q8TB08 M0R2R2
Accessions CAF86729 EAW57441 Q9BZL6 AF151021 AAF36107 AF309082 AAK01149 AK074673 BAC11127 AK075266 BAC11508 AK092896 AK095884 BAG53158 AK300339 BAG62084 AK303629 BAG64637 AL050147 CAB43292 BC015472 AAH15472 BC025307 BC156073 AAI56074 BC156946 AAI56947 DA475821 XM_005258716 XP_005258773 NM_001079880 NP_001073349 NM_001079881 NP_001073350 NM_001079882 NP_001073351 NM_016457 NP_057541
Function Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress- induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin r
Subcellular Location Cytoplasm. Cell membrane {ECO:0000250}. Nucleus. Golgi apparatus, trans-Golgi network. Note=Translocation to the cell membrane is required for kinase activation (By similarity). Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. {ECO:0000250}.
Tissue Specificity Widely expressed.
Top Pathways Rap1 signaling pathway

PKD2 (D1A7) Rabbit mAb - 8188 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1A7
Western blot analysis of extracts from various cell lines using PKD2 (D1A7) Rabbit mAb.

PKD2 (H-6) - sc-374213 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKD2 (H-6): sc-374213. Western blot analysis of PKD2 expression in non-transfected: sc-117752 (A) and mouse PKD2 transfected: sc-127343 (B) 293T whole cell lysates.

PKD2 (F-2) - sc-374344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKD2 (F-2): sc-374344. Western blot analysis of PKD2 expression in non-transfected: sc-117752 (A) and mouse PKD2 transfected: sc-127343 (B) 293T whole cell lysates.

PKD2 (C-19) - sc-74838 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKD2 (C-19): sc-74838. Western blot analysis of PKD2 expression in non-transfected: sc-117752 (A) and mouse PKD2 transfected: sc-127343 (B) 293T whole cell lysates.

PKD2 (E-20) - sc-74839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKD2 (E-20): sc-74839. Western blot analysis of PKD2 expression in non-transfected: sc-117752 (A) and mouse PKD2 transfected: sc-127343 (B) 293T whole cell lysates.

PKD2 (431.2) - sc-100415 from Santa Cruz Biotechnology

Prices $295.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PKD2 (431.2): sc-100415. Western blot analysis of PKD2 expression in HeLa whole cell lysate.

Anti-Protein Kinase D2 (phospho S876) antibody - ab59417 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Protein Kinase D2 (phospho S876) antibody (ab59417) at 1/500 dilutionLane 1 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins)Lane 2 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) with blocking phosphopeptide

Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] - ab51251 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1496Y
All lanes : Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with TPALysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution

Anti-Protein Kinase D2 antibody - ab7281 from Abcam

Prices $400.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab7281 staining Protein Kinase D2 in human BON cells (derived from a human pancreatic carcinoid tumor) by Immunocytochemistry/ Immunofluorescence.BON cells were grown on glass coverslips in 24-well plates. 2 or 3 days after seeding or transfection, cells were treated with vehicle (Me2SO) or PMA (100 nm) for 30 minutes. After treatment, cells were fixed with 4% paraformaldehyde for 20 minutes at 37 °C. After three washes with PBS, the cells were permeabilized with 0.3% Triton X-100 for 15 minutes at 37 °C and blocked with 1% bovine serum albumin/PBS for 10 minutes. The cells were incubated with the primary antibody diluted with 1% bovine serum albumin/PBS for 1 hour at room temperature. Cells were washed three times with PBS and incubated with Alexa-conjugated secondary antibody diluted by 1/500 in 1% bovine serum albumin/PBS. Double staining was performed using antibodies ab7281 and an anti-NT. Co-localization is shown by arrows in the merged images.

Anti-Protein Kinase D2 antibody - ab57114 from Abcam

Prices $382.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Protein Kinase D2 antibody (ab57114) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-Protein Kinase D2 antibody - ab118639 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118639 at 10 µg/ml staining Protein Kinase D2 in Formalin-Fixed, Paraffin-Embedded Human Brain Cerebellum tissue by Immunohistochemistry.

Anti-Protein Kinase D2 antibody - ab59372 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Protein Kinase D2 antibody (ab59372) at 1/500 dilutionLane 1 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins)Lane 2 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins) with immunizing phosphopeptide

Anti-Protein Kinase D2 antibody - ab103559 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed paraffin-embedded section of Human stomach adenocarcinoma. ab103559 used at a dilution of 1/250. Detection: DAB staining.

Anti-Protein Kinase D2 antibody [2E4] - ab117718 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2E4
Immunohistochemistry Image of Formalin-Fixed, Paraffin-Embedded Human Spleen stained with anti-Protein kinase D2 antibody (ab117718) at 10 µg/ml.

Anti-Protein Kinase D2 antibody [3A1] - ab118987 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A1
All lanes : Anti-Protein Kinase D2 antibody [3A1] (ab118987) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Protein Kinase D2 cDNALysates/proteins at 5 µg per lane.

Anti-Protein Kinase D2 antibody [EP1495Y] - ab51250 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1495Y
Anti-Protein Kinase D2 antibody [EP1495Y] (ab51250) at 1/500 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Anti-PRKD2 / PKD2 Antibody (C-Terminus) - LS-C286784 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PKD2 by Immunohistochemistry. Sample: FFPE section of human stomach adenocarcinoma. Antibody: Affinity purified rabbit anti-PKD2 used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-PRKD2 / PKD2 Antibody (aa621-832) - LS-C296118 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant PRKD2 / PKD2.

Anti-PRKD2 / PKD2 Antibody (clone 2E4) IHC-plus™ - LS-B5453 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2E4
Anti-PRKD2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5453 concentration 10 ug/ml.

Anti-PRKD2 / PKD2 Antibody (C-Terminus) - LS-C287009 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PKD2 by Immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PKD1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-PRKD2 / PKD2 Antibody (clone 2E2) - LS-C115567 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E2
Anti-PRKD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.

Anti-PRKD2 / PKD2 Antibody (clone 3A1) - LS-C115601 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.

Anti-PRKD2 / PKD2 Antibody (aa829-878) IHC-plus™ - LS-B5654 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PRKD2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5654 concentration 10 ug/ml.

Anti-PRKD2 / PKD2 Antibody (clone 3F7) - LS-C115588 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F7
Anti-PRKD2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2.

Anti-PRKD2 / PKD2 Antibody (clone 5A3) - LS-C115589 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A3
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody.
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