Anti-Protein Kinase D2 antibody [EP1495Y]
| Name | Anti-Protein Kinase D2 antibody [EP1495Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51250 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1495Y |
| Applications | WB FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide (Human) |
| Description | Rabbit Monoclonal |
| Gene | PRKD2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Protein Kinase D2 antibody [EP1495Y] (ab51250) at 1/500 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17129_ab51250_1.bmp)
Anti-Protein Kinase D2 antibody [EP1495Y] (ab51250) at 1/500 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
Ab51250 (1:100) staining human Protein Kinase D2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.Ab51250 (1:100) staining human Protein Kinase D2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
ICC/IF image of ab51250 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51250, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Protein kinase Cdelta mediates the activation of protein kinase D2 in platelets. - Protein kinase Cdelta mediates the activation of protein kinase D2 in platelets.
Bhavanasi D, Kim S, Goldfinger LE, Kunapuli SP. Biochem Pharmacol. 2011 Oct 1;82(7):720-7.
Protein kinase D2 is a novel regulator of glioblastoma growth and tumor - Protein kinase D2 is a novel regulator of glioblastoma growth and tumor
Azoitei N, Kleger A, Schoo N, Thal DR, Brunner C, Pusapati GV, Filatova A, Genze F, Moller P, Acker T, Kuefer R, Van Lint J, Baust H, Adler G, Seufferlein T. Neuro Oncol. 2011 Jul;13(7):710-24.
Protein kinase D2 is a crucial regulator of tumour cell-endothelial cell - Protein kinase D2 is a crucial regulator of tumour cell-endothelial cell
Azoitei N, Pusapati GV, Kleger A, Moller P, Kufer R, Genze F, Wagner M, van Lint J, Carmeliet P, Adler G, Seufferlein T. Gut. 2010 Oct;59(10):1316-30.