Anti-Protein phosphatase inhibitor 1 antibody [EP902Y]

Name	Anti-Protein phosphatase inhibitor 1 antibody [EP902Y]
Supplier	Abcam
Catalog	ab40877
Prices	$391.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP902Y
Applications	WB IP FC ICC/IF ICC/IF IHC-P
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide within Human Protein phosphatase inhibitor 1 aa 1-100 (N terminal)
Description	Rabbit Monoclonal
Gene	PPP1R1A
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-Protein phosphatase inhibitor 1 antibody [EP902Y] (ab40877) at 1/40000 dilution + Rat brain at 10 µg

Immunohistochemical analysis of paraffin-embedded human normal brain tissue using ab40877 diluted 1:100.Immunohistochemical analysis of paraffin-embedded human normal brain tissue using ab40877 diluted 1:100.

ICC/IF image of ab40877 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40877, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing SH-SY5Y cells stained with ab40877 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40877, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

Product References

Protein phosphatase 1 inhibitor-1 deficiency reduces phosphorylation of renal - Protein phosphatase 1 inhibitor-1 deficiency reduces phosphorylation of renal

Picard N, Trompf K, Yang CL, Miller RL, Carrel M, Loffing-Cueni D, Fenton RA, Ellison DH, Loffing J. J Am Soc Nephrol. 2014 Mar;25(3):511-22.

Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate - Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate

Chao LC, Wroblewski K, Ilkayeva OR, Stevens RD, Bain J, Meyer GA, Schenk S, Martinez L, Vergnes L, Narkar VA, Drew BG, Hong C, Boyadjian R, Hevener AL, Evans RM, Reue K, Spencer MJ, Newgard CB, Tontonoz P. J Lipid Res. 2012 Dec;53(12):2610-9.

The large isoforms of A-kinase anchoring protein 18 mediate the phosphorylation - The large isoforms of A-kinase anchoring protein 18 mediate the phosphorylation

Singh A, Redden JM, Kapiloff MS, Dodge-Kafka KL. Mol Pharmacol. 2011 Mar;79(3):533-40.

Targeting mitotic exit leads to tumor regression in vivo: Modulation by Cdk1, - Targeting mitotic exit leads to tumor regression in vivo: Modulation by Cdk1,

Manchado E, Guillamot M, de Carcer G, Eguren M, Trickey M, Garcia-Higuera I, Moreno S, Yamano H, Canamero M, Malumbres M. Cancer Cell. 2010 Dec 14;18(6):641-54.