Anti-PRPF8 antibody [EPR15229] - C-terminal

• Supplier: Abcam
• Catalog: ab185547
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15229
• Applications: IHC-P FC ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PRPF8 aa 2250 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: PRPF8
• Conjugate: Unconjugated

Product images

All lanes : Anti-PRPF8 antibody [EPR15229] - C-terminal (ab185547) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue sections labeling PRPF8 using ab185547 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling PRPF8 using ab185547 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Flow Cytometry analysis of K562 cells labeling PRPF8 using ab185547 at a 1/190 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.