PRPF8

Gene Symbol PRPF8
Entrez Gene 10594
Alt Symbol HPRP8, PRP8, PRPC8, RP13, SNRNP220
Species Human
Gene Type protein-coding
Description pre-mRNA processing factor 8
Other Description 220 kDa U5 snRNP-specific protein|PRP8 homolog|PRP8 pre-mRNA processing factor 8 homolog|U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p|apoptosis-regulated protein 1|apoptosis-regulated protein 2|p220|pre-mRNA-processing-splicing factor 8|precursor mRNA processing protein|splicing factor Prp8
Swissprots Q6P2Q9 O75965 O14547
Accessions EAW90588 EAW90589 EAW90590 EAW90591 EAW90592 EAW90593 EAW90594 Q6P2Q9 AB007510 BAA22563 AF092565 AAC61776 AK058034 AK126169 AK222905 BAD96625 AK296344 BAG59028 AY486134 AAS64747 AY486135 AAS64748 BC000579 BC034545 BC064370 AAH64370 BX649099 CD698473 DB081408 NM_006445 NP_006436
Function Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. {ECO:0000269|PubMed:20595234, ECO:0000303|PubMed:15840809}.
Subcellular Location Nucleus speckle {ECO:0000250}.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:10411133}.
Top Pathways Spliceosome

PRP8 (F-6) - sc-55534 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PRP8 (F-6): sc-55534. Western blot analysis of PRP8 expression in Y79 nuclear extract (A) and CTLL-2 (B) and KNRK (C) whole cell lysates.

PRP8 (C-18) - sc-23647 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PRP8 (C-18): sc-23647. Western blot analysis of PRP8 expression in K-562 (A), Jurkat (B), HeLa (C) and HL-60 (D) nuclear extracts.

PRP8 (E-5) - sc-55533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PRP8 (E-5): sc-55533. Western blot analysis of PRP8 expression in Y79 nuclear extract (A) and CTLL-2 (B) and KNRK (C) whole cell lysates.

PRP8 (H-300) - sc-30207 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PRP8 (H-300): sc-30207. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-PRPF8 antibody - ab79237 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRPF8 antibody (ab79237) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PRPF8 antibody - ab87433 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRPF8 antibody (ab87433) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PRPF8 antibody - ab157114 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRPF8 antibody (ab157114) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgLane 5 : NIH 3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-PRPF8 antibody [2834C1a] - ab51366 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2834C1a
IHC image of PRPF8 staining in Human Normal Cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51366, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PRPF8 antibody [EPR15228] - ab190347 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15228
All lanes : Anti-PRPF8 antibody [EPR15228] (ab190347) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Anti-PRPF8 antibody [EPR15229] - C-terminal - ab185547 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15229
All lanes : Anti-PRPF8 antibody [EPR15229] - C-terminal (ab185547) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PRPF8 antibody - C-terminal - ab137694 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PRPF8 antibody - C-terminal (ab137694) at 1/500 dilution + whole cell lysate prepared from Raji cells at 30 µg

Anti-PRPF8 Antibody - LS-C75250 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-PRPF8 Antibody (aa2285-2335) - LS-C290569 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse PRPF8 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-PRPF8 Antibody (aa1825-1875) - LS-C290568 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse PRPF8 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PRPF8 Antibody (aa850-900) - LS-C290567 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse PRPF8 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PRPF8 Antibody (aa360-409) - LS-C110549 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PRPF8 antibody LS-C110549 Western blot of Jurkat lysate.

Anti-PRPF8 Antibody - LS-C334488 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using PRPF8 antibody.

Anti-PRPF8 Antibody (clone 2834C1a) - LS-C342473 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2834C1a
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PRPF8 mAb.

Anti-PRPF8 Antibody (clone 2834C1a) - LS-C184431 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2834C1a
Immunofluorescent staining using PRPF8 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-PRPF8 antibody.

PRPF8 Antibody - NBP2-22274 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRPF8 Antibody [NBP2-22274] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (5 ug) cells. NBP2-22274 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

PRPF8 Antibody - NBP2-22273 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRPF8 Antibody [NBP2-22273] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

PRPF8 Antibody - NBP2-22272 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRPF8 (C-terminal) antibody produced in rabbit - SAB2700372 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PRPF8 (C-terminal): <B>Cat. No. SAB2700372</B>: Western Blot analysis of PRPF8 in extracts from <B>Lanes</B><BR/>A. Raji using <B>Cat. No. SAB2700372</B> at a 1:500 dilution.

Anti-PRPF8 antibody produced in rabbit - SAB2101885 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PRPF8: <B>Cat. No.SAB2101885</B>: PRPF8 in Jurkat cell lysate with PRPF8 antibody at 1.0 μg/mL.

PRPF8 antibody - N-terminal region (ARP40649_P050) - ARP40649_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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