ICC/IF image of ab12097 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12097 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Performed under reducing conditions.
Performed under reducing conditions.
Performed under reducing conditions.
ab12097 PSD staining (10µg/ml) on mouse brain (5µm sagittal section on slide; 4% PFA perfused & paraffin embedded). Staining observed as expected in the hippocampus, frontal cortex and cortex with ab12097. Blue=haemotoxylin nuclear counter-stain.
![ab12097 PSD staining (10µg/ml) on mouse brain (5µm sagittal section on slide; 4% PFA perfused & paraffin embedded). Granular staining observed as expected in [A] cerebellum and [B] hippocampus with ab12097. Blue=haemotoxylin nuclear counter-stain. Images taken with X10 objective.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17635_ab12097_3.jpg)
ab12097 PSD staining (10µg/ml) on mouse brain (5µm sagittal section on slide; 4% PFA perfused & paraffin embedded). Granular staining observed as expected in [A] cerebellum and [B] hippocampus with ab12097. Blue=haemotoxylin nuclear counter-stain. Images taken with X10 objective.