Anti-PSD95 antibody

• Supplier: Abcam
• Catalog: ab12093
• Prices: $391.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide derived from residues 1 - 100 of Mouse PSD95
• Blocking Peptide: Mouse PSD95 peptide
• Description: Goat Polyclonal
• Gene: DLG4
• Conjugate: Unconjugated

Product images

All lanes : Anti-PSD95 antibody (ab12093) at 2 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lysates/proteins at 25 µg per lane.SecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/5000 dilutionPerformed under reducing conditions.

Lane 1 : Anti-PSD95 antibody (ab12093) at 0.3 µg/mlLane 2 : Anti-PSD95 antibody (ab12093) at 1 µg/mlLanes 3 - 4 : Anti-PSD95 antibody (ab12093) at 3 µg/mlLane 1 : 10µg mouse brain extract/laneLane 2 : 10µg mouse brain extract/laneLane 3 : 10µg mouse brain extract/laneLane 4 : 10µg mouse brain extract/lane with Mouse PSD95 peptide (ab12419) at 1 µg/mlPerformed under reducing conditions.

ab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm.

ab12093 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde and blocked with 10% donkey serum in  0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab12093 at 1/500 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-goat polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 expressed in exon, dendrites and particularly carrying in the dendritic spinesSee Abreview

All lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilutionLane 1 : rat cortical lysateLane 2 : mouse cortical lysateLane 3 : BA21 human cortical lane lysateLane 4 : BA21 human cortical lane lysateLane 5 : BA21 human cortical lane lysateLane 6 : BA21 human cortical lane lysateLane 7 : BA21 human cortical lane lysateLane 8 : BA21 human cortical lane lysateLysates/proteins at 20 µg per lane.SecondaryAlexafluor 680Performed under reducing conditions.

Product References

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