![All lanes : Anti-PSIP1 antibody [6E4] (ab110023) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : Cos7 cell lysateLane 5 : PC-12 cell lysateLane 6 : Hela cell lysateLane 7 : NIH3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17752_PSIP1-Primary-antibodies-ab110023-1.jpg)
All lanes : Anti-PSIP1 antibody [6E4] (ab110023) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : Cos7 cell lysateLane 5 : PC-12 cell lysateLane 6 : Hela cell lysateLane 7 : NIH3T3 cell lysate
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110023 at a dilution of 1/200 with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using ab110023 at a dilution of 1/200 with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using ab110023 at a dilution of 1/200 with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110023 at a dilution of 1/200 with DAB staining.
Immunofluorescence analysis of NIH3T3 cells using ab110023 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
![Overlay histogram showing SH-SY5Y cells stained with ab110023 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110023, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17758_PSIP1-Primary-antibodies-ab110023-8.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab110023 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110023, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.