All lanes : Anti-PSMB10 antibody [EPR14902] (ab183506) at 1/1000 dilutionLane 1 : Human fetal spleen tissue lysateLane 2 : Human fetal thymus tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
All lanes : Anti-PSMB10 antibody [EPR14902] (ab183506) at 1/5000 dilutionLane 1 : HuT 78 cell line lysateLane 2 : Raji cell line lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution