PSMB10

Gene Symbol PSMB10
Entrez Gene 5699
Alt Symbol LMP10, MECL1, beta2i
Species Human
Gene Type protein-coding
Description proteasome (prosome, macropain) subunit, beta type, 10
Other Description low molecular mass protein 10|macropain subunit MECl-1|multicatalytic endopeptidase complex subunit MECl-1|proteasome MECl-1|proteasome catalytic subunit 2i|proteasome subunit MECL1|proteasome subunit beta 7i|proteasome subunit beta type-10|proteasome subunit beta-2i
Swissprots Q5U098 P40306 B2R5J4
Accessions CAA50709 CBX46811 EAW83187 P40306 AK225658 AK298012 BAG60316 AK312208 BAG35141 BC017198 AAH17198 BC052369 AAH52369 BM845639 BM981173 BP289065 BT019723 AAV38528 BT019724 AAV38529 CR456982 CAG33263 Y13640 CAA73982 NM_002801 NP_002792
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Subcellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus {ECO:0000250}.
Top Pathways Proteasome

MECL-1 (C-2) - sc-133236 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MECL-1 (C-2): sc-133236. Western blot analysis of MECL-1 expression in HL-60 (A) and Jurkat (B) whole cell lysates.

MECL-1 (E-20) - sc-18170 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MECL-1 (E-20): sc-18170. Western blot analysis of MECL-1 expression in non-transfected: sc-117752 (A) and mouse MECL-1 transfected: sc-121580 (B) 293T whole cell lysates.

MECL-1 (H-70) - sc-50425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MECL-1 (H-70): sc-50425. Western blot analysis of MECL-1 expression in human placenta (A) and mouse liver (B) tissue extracts.

Anti-PSMB10 antibody - ab77735 from Abcam

Prices $388.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
IHC image of PSMB10 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77735, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PSMB10 antibody - ab106922 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab106922 (5µg/ml) staining of paraffin embedded Human Tonsil following teamed antigen retrieval with citrate buffer pH 6 and AP-staining shows defined staining in a subset of cells.

Anti-PSMB10 antibody - ab94623 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PSMB10 antibody (ab94623) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PSMB10 antibody - ab112947 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PSMB10 antibody (ab112947) at 1/100 dilution + MCF7 cell line lysate at 35 µg

Anti-PSMB10 antibody [EPR14902] - ab183506 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14902
All lanes : Anti-PSMB10 antibody [EPR14902] (ab183506) at 1/1000 dilutionLane 1 : Human fetal spleen tissue lysateLane 2 : Human fetal thymus tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Anti-PSMB10 antibody [EPR14903] - ab190790 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14903
All lanes : Anti-PSMB10 antibody [EPR14903] (ab190790) at 1/1000 dilutionLane 1 : Human fetal thymus lysateLane 2 : Daudi cell lysateLane 3 : Raji cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-PSMB10 Antibody (Internal) IHC-plus™ - LS-B10305 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-PSMB10 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-PSMB10 Antibody (aa213-242, Biotin) - LS-C216999 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa213-242, Azide-free, HRP) - LS-C217000 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa213-242, PE) - LS-C217001 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa213-242, FITC) - LS-C221188 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa213-242) - LS-C324356 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa1-274, Azide-free) - LS-C328334 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-PSMB10 Antibody - LS-C341799 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa143-192) - LS-C111927 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PSMB10 antibody LS-C111927 Western blot of Placenta lysate.

Anti-PSMB10 Antibody (aa213-242) - LS-C162228 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PSMB10 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The PSMB10 antibody detected the PSMB10 protein (arrow).

Anti-PSMB10 Antibody - LS-C346083 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of HeLa cell using PSMB10 antibody. Blue: DAPI for nuclear staining.

Anti-PSMB10 Antibody (clone 3F8) - LS-C198017 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F8
Immunofluorescence of monoclonal antibody to PSMB10 on HeLa cell. [antibody concentration 10 ug/ml]

Anti-PSMB10 Antibody (aa213-242, AP) - LS-C216997 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSMB10 Antibody (aa213-242, APC) - LS-C216998 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

PSMB10/MECL1 Antibody - NBP1-58937 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PSMB10 Antibody [NBP1-58937] - Human Placenta lysate, concentration 0.2-1 ug/ml.

PSMB10/MECL1 Antibody - AF7684 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, HuT 78 human cutaneous T cell lymphoma cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PSMB10/MECL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PSMB10/MECL1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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