All lanes : Anti-PSMB10 antibody [EPR14903] (ab190790) at 1/1000 dilutionLane 1 : Human fetal thymus lysateLane 2 : Daudi cell lysateLane 3 : Raji cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PSMB10 with ab190790 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor555) secondary antibody at 1/200 dilution. Counter stained with DAPI.