All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilutionLane 1 : Human prostate cancer whole tissue lysateLane 2 : Human prostate cancer whole tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.