PSME1

Gene Symbol PSME1
Entrez Gene 5720
Alt Symbol IFI5111, PA28A, PA28alpha, REGalpha
Species Human
Gene Type protein-coding
Description proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Other Description 11S regulator complex subunit alpha|29-kD MCP activator subunit|IGUP I-5111|activator of multicatalytic protease subunit 1|interferon gamma up-regulated I-5111 protein|interferon-gamma IEF SSP 5111|interferon-gamma-inducible protein 5111|proteasome activator complex subunit 1
Swissprots A6NJG9 Q06323 H0YNE3 Q9UEF4 Q6IBM2
Accessions AAA53230 AAF02217 BAA28836 EAW66105 EAW66106 Q06323 AI445328 AK307150 AK308572 AK311490 AK312211 BAG35144 BC000352 AAH00352 BC007503 AAH07503 BJ993879 BM470561 BM981616 BQ060125 BT019337 AAV38144 BU192084 BU731923 BX248293 CAD62621 CR456780 CAG33061 CR541658 CAG46459 DQ185047 DQ891838 ABM82764 DQ895024 ABM85950 L07633 AAA16521 NM_001281528 NP_001268457 NM_001281529 NP_001268458 NM_006263 NP_006254 NM_176783 NP_788955
Function Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Top Pathways Proteasome, Antigen processing and presentation

PA28α (D1C10) Rabbit mAb - 9643 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1C10
Western blot analysis of extracts from various cell lines using PA28α (D1C10) Rabbit mAb.

PA28α Antibody - 2408 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7, Neuro2A, C6, CHO and COS cells using PA28α Antibody.

PA28α (D4F12) Rabbit mAb - 8657 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4F12
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with Myc/DDK-tagged cDNA expression constructs encoding full-length human PA28α (hPA28α; +), full-length human PA28β (hPA28β; +), or full-length human PA28γ (hPA28γ; +), using PA28α (D4F12) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody) #2368 (lower).

PA28α (L-18) - sc-21267 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PA28α (L-18): sc-21267. Western blot analysis of PA28α expression in A549 (A), NCI-H1299 (B), HEK293 (C), A431 (D), HL-60 (E) and HeLa (F) whole cell lysates.

Anti-PSME1 antibody - ab104098 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab104098 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104098 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PSME1 antibody - ab89620 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PSME1 antibody (ab89620) at 1 µg/ml + A431 cell lysate at 50 µg

Anti-PSME1 antibody [EPR10967(B)] - ab155091 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10967(B)
All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-PSME1 antibody [EPR10968(B)] - ab155985 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10968(B)
All lanes : Anti-PSME1 antibody [EPR10968(B)] (ab155985) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : MCF-7 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-PSME1 Antibody (C-Terminus) - LS-C38020 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (Lys13) - LS-C38022 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody - LS-C86227 from LifeSpan Bioscience

Prices $755.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258, APC) - LS-C267342 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258, AP) - LS-C267343 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258, Biotin) - LS-C267344 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258, FITC) - LS-C267345 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258, Azide-free, HRP) - LS-C267347 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258, PE) - LS-C267349 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (aa219-258) - LS-C324370 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody - LS-C341589 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME1 Antibody (clone 1G11) - LS-C179480 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G11

Anti-PSME1 Antibody - LS-C334008 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat testis using PSME1 antibody at dilution of 1:200 (400x lens).

Anti-PSME1 Antibody (C-Terminus) - LS-C55331 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PSME1 Antibody (aa1-50) - LS-C290507 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-PSME1 Antibody (aa219-258) - LS-C162248 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PSME1 Antibody IHC of formalin-fixed and paraffin-embedded hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PSME1 Antibody (aa72-121) IHC-plus™ - LS-B8233 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.
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