All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilutionLane 1 : Human fetal liver lysateLane 2 : Jurkat cell lysateLane 3 : Daudi cell lysateLane 4 : A549 cell lysateLane 5 : BxPC3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with ab133734 at a 1/100 dilution.
Immunofluorescent staining of PTBP1 in A549 cells, using ab133734 at a 1/250 dilution.