PTBP1

Gene Symbol PTBP1
Entrez Gene 5725
Alt Symbol HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB
Species Human
Gene Type protein-coding
Description polypyrimidine tract binding protein 1
Other Description 57 kDa RNA-binding protein PPTB-1|RNA-binding protein|heterogeneous nuclear ribonucleoprotein I|heterogeneous nuclear ribonucleoprotein polypeptide I|hnRNP I|polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I)|polypyrimidine tract-binding protein 1
Swissprots Q9BUQ0 P26599
Accessions AAC99798 EAW61152 EAW61153 EAW61154 EAW61155 EAW61156 EAW61157 EAW61158 P26599 AB208910 BAD92147 BC002397 AAH02397 BC004383 AAH04383 BC013694 AAH13694 BC023219 BQ068293 BT006819 AAP35465 X60648 CAA43056 X62006 CAA43973 X65371 CAA46443 X65372 CAA46444 X66975 CAA47386 XM_005259597 XP_005259654 XM_005259598 XP_005259655 XR_244034 XR_244035 NM_002819 NP_002810 NM_031990 NP_114367 NM_031991 NP_114368 NM_175847
Function Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. {ECO:0000269|PubMed:11003644, ECO:0000269|PubMed:15009664, ECO:0000269|PubMed:16179478, ECO:0000269|PubMed:16260624, ECO:0000269|PubMed:21518792}.
Subcellular Location Nucleus.

PTBP1 Antibody - 8776 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SW620 and HT-29 cells using PTBP1 Antibody.

hnRNP I (N-20) - sc-16547 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hnRNP I (N-20): sc-16547. Western blot analysis of hnRNP I expression in HeLa whole cell lysate.

hnRNP I (SH54) - sc-56701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hnRNP I (SH54): sc-56701. Western blot analysis of hnRNP I expression in MEG-01 (A), HeLa (B) and A549 (C) nuclear extracts and SHP-77 (D) and HeLa (E) whole cell lysates.

hnRNP I (L-14) - sc-16549 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hnRNP I (L-14): sc-16549. Western blot analysis of hnRNP I expression in MEG-01 (A) and Jurkat (B) nuclear extracts.

hnRNP I (3H7) - sc-73391 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
hnRNP I (3H7): sc-73391. Western blot analysis of hnRNP I expression in MEG-01 (A), HeLa (B) and A549 (C) nuclear extracts and SHP-77 (D) and HeLa (E) whole cell lysates.

Anti-PTBP1 antibody [3H8] - ab119285 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3H8
Anti-PTBP1 antibody [3H8] (ab119285) + A431 cell lysate

Anti-PTBP1 antibody [EPR9048(B)] - ab133734 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9048(B)
All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilutionLane 1 : Human fetal liver lysateLane 2 : Jurkat cell lysateLane 3 : Daudi cell lysateLane 4 : A549 cell lysateLane 5 : BxPC3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-PTBP1 antibody [EPR9048(B)] (Alexa Fluor® 647) - ab201020 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9048(B)
ab201020 staining PTBP1 in A549 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201020 at 1/10000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). This product also gave a positive signal under the same testing conditions in A549 cells fixed with 100% methanol (5min).

Anti-PTBP1 antibody [EPR9049(B)] - ab134950 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9049(B)
Immunofluorescent analysis of HeLa cells labelling PTBP1 with ab134950 at 1/100 dilution.

Anti-PTBP1 antibody [SH54] - ab30317 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SH54
Overlay histogram showing HeLa cells stained with ab30317 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30317, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-PTBP1 antibody - N-terminal - ab156226 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PTBP1 antibody - N-terminal (ab156226) at 1/100 dilution + MCF7 cell line lysates at 35 µg

Anti-PTBP1 antibody - ab58131 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
PTBP1 antibody (ab58131) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.

Anti-PTBP1 Antibody (N-Terminus) - LS-C30442 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Daudi Cell Lysate

Anti-PTBP1 Antibody (clone 1D4B) - LS-C189751 from LifeSpan Bioscience

Prices $345.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal
Clone 1D4B

Anti-PTBP1 Antibody (aa26-55, AP) - LS-C267366 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa26-55, APC) - LS-C267367 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa26-55, Biotin) - LS-C267368 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa26-55, FITC) - LS-C267369 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa26-55, Azide-free, HRP) - LS-C267370 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa26-55, PE) - LS-C267371 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (C-Terminus) - LS-C101841 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PTBP1 antibody LS-C101841 Western blot of Jurkat lysate.

Anti-PTBP1 Antibody (aa26-55) - LS-C324376 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa45-145, Azide-free) - LS-C328286 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-PTBP1 Antibody - LS-C341352 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PTBP1 Antibody (aa26-55) - LS-C155877 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PTBP1 Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The PTBP1 antibody detected the PTBP1 protein (arrow).
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