Anti-PTBP1 antibody [SH54]
| Name | Anti-PTBP1 antibody [SH54] |
|---|---|
| Supplier | Abcam |
| Catalog | ab30317 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | SH54 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | HeLa nuclear extract (Human) |
| Description | Mouse Monoclonal |
| Gene | PTBP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab30317 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30317, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/18217_PTBP1-Primary-antibodies-ab30317-1.jpg)
Overlay histogram showing HeLa cells stained with ab30317 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30317, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Roles of polypyrimidine tract binding proteins in major immediate-early gene - Roles of polypyrimidine tract binding proteins in major immediate-early gene
Cosme RS, Yamamura Y, Tang Q. J Virol. 2009 Apr;83(7):2839-50.
The perinucleolar compartment and transcription. - The perinucleolar compartment and transcription.
Huang S, Deerinck TJ, Ellisman MH, Spector DL. J Cell Biol. 1998 Oct 5;143(1):35-47.
The dynamic organization of the perinucleolar compartment in the cell nucleus. - The dynamic organization of the perinucleolar compartment in the cell nucleus.
Huang S, Deerinck TJ, Ellisman MH, Spector DL. J Cell Biol. 1997 Jun 2;137(5):965-74.