ab2614 staining PTIP in mouse embryonic fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 in PBS and blocked with 20% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/300) for 18 hours at 4°C. An Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Lanes 1 - 3 : Anti-PTIP antibody (ab2614) at 1/250 dilutionLanes 4 - 6 : Anti-PTIP antibody (ab2614) at 1/500 dilutionLanes 7 - 9 : Anti-PTIP antibody (ab2614) at 1/1000 dilutionLane 1 : 30 ug LCL1 whole cell lysate.Lane 2 : 30 ug LCL2 whole cell lysate.Lane 3 : 30 ug LCL3 whole cell lysate.Lane 4 : 30 ug LCL1 whole cell lysate.Lane 5 : 30 ug LCL2 whole cell lysate.Lane 6 : 30 ug LCL3 whole cell lysate.Lane 7 : 30 ug LCL1 whole cell lysate.Lane 8 : 30 ug LCL2 whole cell lysate.Lane 9 : 30 ug LCL3 whole cell lysate.Performed under reducing conditions.