Lanes 1, 4 & 7 : Anti-PTIP antibody (ab70434) at 0.25 µg/mlLanes 2, 5 & 8 : Anti-PTIP antibody (ab70434) at 0.1 µg/mlLanes 3, 6 & 9 : Anti-PTIP antibody (ab70434) at 0.025 µg/mlLane 1 : Whole cell lysate from 293T cells at 5 µgLane 2 : Whole cell lysate from 293T cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 50 µgLane 4 : Whole cell lysate from 293T cells at 5 µgLane 5 : Whole cell lysate from 293T cells at 15 µgLane 6 : Whole cell lysate from 293T cells at 50 µgLane 7 : Whole cell lysate from 293T cells at 5 µgLane 8 : Whole cell lysate from 293T cells at 15 µgLane 9 : Whole cell lysate from 293T cells at 50 µgdeveloped using the ECL technique
30ug of whole cell lysate from 293T cells were immunoprecipitated using ab70434 at 10, 5 and 1ug/mg of lysate respectively in lanes 1, 2 and 3. For the subsequent blot ab70434 was used at 0.25ug/ml.