All lanes : Anti-PTP lambda antibody (ab13223) at 1/500 dilutionLane 1 : Human brain lysateLane 2 : Human kidney lysateLane 3 : Human pancreas lysateLane 4 : Human heart lysateLane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/mlLane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/mlLane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/mlLane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutionPerformed under reducing conditions.Exposure time : 5 minutesA weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).
Image courtesy of www.proteinatlas.orgab13223 staining PTP lambda in human cerebral cortex and showing a strong staining on the neuronal cells. Paraffin embedded human cerebral cortex tissues were cut into 4µm sections and incubated with ab13223 (1/75) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab13223 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab13223 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab13223, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.