ab5419, at a 1/50 dilution, staining paraffin embedded human brain tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.
All lanes : Anti-PTP1B (phospho S50) antibody (ab59419) at 1/2000 dilutionLane 1 : Whole tissue lysate prepared from human muscleLane 2 : Whole tissue lysate prepared from mouse heartLysates/proteins at 75 µg per lane.SecondaryHRP conjugated goat anti-rabbit polyclonal at 1/10000 dilutiondeveloped using the ECL techniqueObserved band size : 50 kDa (why is the actual band size different from the predicted?)Exposure time : 2 minutesImage courtesy of an anonymous Abreview.See Abreview