Anti-PTP1B antibody
| Name | Anti-PTP1B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2009 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide derived from residues 350 to the C-terminus of Human PTP1B |
| Description | Rabbit Polyclonal |
| Gene | PTPN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PTP1B antibody (ab2009) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab2009 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab2009, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
Integrated effects of leptin in the forebrain and hindbrain of male rats. - Integrated effects of leptin in the forebrain and hindbrain of male rats.
Desai BN, Harris RB. Endocrinology. 2013 Aug;154(8):2663-75.
Steroid effects on ZAP-70 and SYK in relation to apoptosis in poor prognosis - Steroid effects on ZAP-70 and SYK in relation to apoptosis in poor prognosis
Boelens J, Lust S, Van Bockstaele F, Van Gele M, Janssens A, Derycke L, Vanhoecke B, Philippe J, Bracke M, Offner F. Leuk Res. 2009 Oct;33(10):1335-43.
Mechanistic insight into taxol-induced cell death. - Mechanistic insight into taxol-induced cell death.
Impens F, Van Damme P, Demol H, Van Damme J, Vandekerckhove J, Gevaert K. Oncogene. 2008 Jul 31;27(33):4580-91.