Anti-PTP1B antibody
| Name | Anti-PTP1B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59373 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic non-phosphopeptide derived from Human PTP1B around the phosphorylation site of serine 50 (D-V-SP-P-F) |
| Description | Rabbit Polyclonal |
| Gene | PTPN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ab59373 at a 1/50-1/100 dilution.Left image: un-treated.Right image: treated with immunizing peptide.
ICC/IF image of ab59373 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59373, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.