Anti-PTRF antibody
| Name | Anti-PTRF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab48824 |
| Prices | $359.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Mouse, Rat, Human, Monkey, Hamster, Dog |
| Antigen | A region within synthetic peptide: PEKEGDELGE GERPEDDTAA IELSSDEAVE VEEVIEESRA ERIKRSGLRR, corresponding to internal sequence amino acids 181-230 of Mouse PTRF Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | PTRF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PTRF antibody (ab48824) at 1/2000 dilution + Rat C6 cells, whole cell lysate at 25 µgSecondaryHRP conjugated goat anti-rabbit antibody at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Lane 1 : Anti-PTRF antibody (ab48824) at 5 µg/mlLane 2 : Anti-PTRF antibody (ab48824) at 2.5 µg/mlLane 3 : Anti-PTRF antibody (ab48824) at 1.25 µg/mlLane 1 : Cell lysate prepared from mouse NIH3T3 cellsLane 2 : Cell lysate prepared from mouse NIH3T3 cellsLane 3 : Cell lysate prepared from mouse NIH3T3 cellsLysates/proteins at 25 µg per lane.
ab48824 (4µg/ml) staining PTRF in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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