PTRF

Gene Symbol PTRF
Entrez Gene 284119
Alt Symbol CAVIN, CAVIN1, CGL4, FKSG13, cavin-1
Species Human
Gene Type protein-coding
Description polymerase I and transcript release factor
Other Description RNA polymerase I and transcript release factor|TTF-I interacting peptide 12
Swissprots Q9HAP4 B2RAW7 Q96H74 Q9BT85 Q6NZI2 Q6GMY1 O00535
Accessions EAW60828 Q6NZI2 AF000421 AAC63404 AF312393 AAG27093 AK000715 AK074512 AK092406 BAG52547 AK298072 BAG60361 AK298563 BAG60757 AK314389 BAG37014 AL050224 BC004295 AAH04295 BC008849 AAH08849 BC066123 AAH66123 BC073759 AAH73759 XM_005257242 XP_005257299 NM_012232 NP_036364
Function Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. {ECO:0000269|PubMed:18056712, ECO:0000269|PubMed:18191225, ECO:0000269|PubMed:19726876}.
Subcellular Location Membrane, caveola. Cell membrane {ECO:0000269|PubMed:15242332, ECO:0000269|PubMed:17026959}. Microsome {ECO:0000269|PubMed:15242332, ECO:0000269|PubMed:17026959}. Endoplasmic reticulum. Cytoplasm, cytosol. Mitochondrion {ECO:0000269|PubMed:15242332, ECO:0000269|PubMed:17026959}. Nucleus {ECO:0000269|PubMed:15242332, ECO:0000269|PubMed:17026959}. Note=Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co- localizes with CAV1 in lipid rafts in adipocytes. {ECO:0000250}.

PTRF (C-20) - sc-82326 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PTRF (F-20) - sc-82327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PTRF (H-55) - sc-366701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-PTRF antibody - ab48824 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PTRF antibody (ab48824) at 1/2000 dilution + Rat C6 cells, whole cell lysate at 25 µgSecondaryHRP conjugated goat anti-rabbit antibody at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PTRF antibody - ab78553 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PTRF antibody (ab78553) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PTRF antibody - ab76919 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PTRF antibody (ab76919) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Anti-PTRF antibody - ab134320 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast (adipocytes) tissue labelling PTRF with ab134320 at 5 µg/ml, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PTRF antibody - ab83912 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PTRF antibody - ab58019 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
PTRF antibody (ab58019) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human pancreas.

Anti-PTRF antibody - C-terminal - ab181343 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-PTRF antibody - C-terminal (ab181343) at 0.5 µg/mlLane 2 : Anti-PTRF antibody - C-terminal (ab181343) at 1 µg/mlLane 1 : Human spleen tissue lysateLane 2 : Human spleen tissue lysate

Anti-PTRF antibody - N-terminal - ab135655 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab135655 stained A549 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab135655 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PTRF / CAVIN Antibody (aa150-200) - LS-C91659 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-PTRF / CAVIN Antibody (aa238-288) - LS-C91660 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-PTRF / CAVIN Antibody (C-Terminus) - LS-C209363 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PTRF / CAVIN Antibody (aa150-200) - LS-C288077 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PTRF / CAVIN Antibody (aa125-175) - LS-C288079 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PTRF / CAVIN Antibody (aa238-288) - LS-C288080 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PTRF / CAVIN Antibody (clone 1F7) - LS-C133852 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F7
Immunoperoxidase of monoclonal antibody to PTRF on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

Anti-PTRF / CAVIN Antibody - LS-C335625 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using PTRF antibody.

Anti-PTRF / CAVIN Antibody (C-Terminus) - LS-C100621 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-PTRF Antibody antibody in A375 cell line lysates (35 ug/lane). PTRF(arrow) was detected using the purified antibody.

Anti-PTRF / CAVIN Antibody (aa214-263) - LS-C206114 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PTRF / CAVIN antibody Western Blot of MCF7. Antibody dilution: 1 ug/ml.

Anti-PTRF / CAVIN Antibody (N-Terminus) IHC-plus™ - LS-B7197 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PTRF antibody IHC of human breast, adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7197 concentration 5 ug/ml.

Anti-PTRF / CAVIN Antibody (aa177-226) IHC-plus™ - LS-B5629 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PTRF antibody IHC of mouse vessel and adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5629 concentration 5 ug/ml.

Anti-PTRF / CAVIN Antibody (C-Terminus) IHC-plus™ - LS-B10088 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PTRF / CAVIN antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10088 concentration 10 ug/ml.

PTRF Antibody - NB100-60635 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PTRF Antibody [NB100-60635] - Detection of Human and Mouse PTRF on HeLa whole cell lysate using NB100-60635. PTRF was also immunoprecipitated by rabbit anti-PTRF antibodies NB100-60633 and NB100-60634.
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