Ab66059 was coated on to an ELISA plate at a concentration of 0.01 mg/ml. Afer blocking, the immunogen (recombinant tagged fragment) was added to the wells at a range of concentrations (0.01-1000 ng/ml). A tag specific secondary antibody was added for detection at a concentration of 1 µg/ml.
Anti-PU.1/Spi1 antibody (ab66059) at 1/500 dilution + Immunogen (recombinant fragment) at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilutiondeveloped using the ECL technique
All lanes : Anti-PU.1/Spi1 antibody (ab66059) at 1/500 dilutionLane 1 : PU.1/Spi1 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilutiondeveloped using the ECL technique