SPI1

Gene Symbol SPI1
Entrez Gene 6688
Alt Symbol OF, PU.1, SFPI1, SPI-1, SPI-A
Species Human
Gene Type protein-coding
Description Spi-1 proto-oncogene
Other Description 31 kDa transforming protein|31 kDa-transforming protein|hematopoietic transcription factor PU.1|spleen focus forming virus (SFFV) proviral integration oncogene spi1|transcription factor PU.1
Swissprots P17947
Accessions AAC50247 CDL93508 EAW67924 EAW67925 EAW67926 P17947 AK310065 BC111379 AAI11380 BI519776 BI909430 BM561863 BP271276 CD368431 HG503768 HG503769 HG503770 X52056 CAA36281 XM_011520305 XP_011518607 XM_011520306 XP_011518608 XM_011520307 XP_011518609 XM_011520308 XP_011518610 NM_001080547 NP_001074016 NM_003120 NP_003111
Function Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237, ECO:0000269|PubMed:23166356}.
Top Pathways Acute myeloid leukemia, Osteoclast differentiation, HTLV-I infection, Epstein-Barr virus infection, Transcriptional misregulation in cancer

PU.1 (9G7) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 2240 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 9G7
Flow cytometric analysis of MCF-7 cells (blue) or THP-1 cells (green) using PU.1 (9G7) Rabbit mAb (Alexa Fluor ® 647 Conjugate).

PU.1 (9G7) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 2216 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 9G7
Flow cytometric analysis of MCF-7 cells (blue) or THP-1 cells (green) using PU.1 (9G7) Rabbit mAb (Alexa Fluor ® 488 Conjugate).

PU.1 Antibody - 2266 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from RAW, p388D1, WEHI-3 and THP1 cells using #2266.

PU.1 (9G7) Rabbit mAb - 2258 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 9G7
Western blot analysis of extracts from RAW, THP1 and p388D1 cells using PU.1 (9G7) Rabbit mAb.

PU.1 (A-7) - sc-365208 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PU.1 (A-7): sc-365208. Western blot analysis of PU.1 expression in K-562 nuclear extract.

PU.1 (C-3) - sc-390405 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PU.1 (C-3): sc-390405. Western blot analysis of PU.1 expression in K-562 (A) and RAW 264.7 (B) nuclear extracts.

PU.1 (Spi-1) (B-9) - sc-390659 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PU.1 (Spi-1) (B-9): sc-390659. Western blot analysis of PU.1 expression in K-562 nuclear extract.

PU.1 (G-11) - sc-390618 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PU.1 (G-11): sc-390618. Western blot analysis of PU.1 expression in RAW 264.7 nuclear extract.

PU.1 (H-135) - sc-22805 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PU.1 (H-135): sc-22805. Western blot analysis of PU.1 expression in NIH/3T3 (A) and MM-142 (B) nuclear extracts and NIH/3T3 whole cell lysate (C).

PU.1 (Spi-1) (T-21) - sc-352 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PU.1 (Spi-1) (T-21): sc-352. Western blot analysis of PU.1 expression in K-562 nuclear extract.

PU.1 (N-19) - sc-5972 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PU.1 (D-19) - sc-5949 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PU.1 (D-19): sc-5949. Western blot analysis of PU.1 expression in Hep G2 (A) and WiDR (B) whole cell lysates.

Anti-PU.1/Spi1 antibody - ab88082 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ab88082, at 10 µg/ml, staining PU.1/Spi1 in HeLa cells by Immunofluorescence.

Anti-PU.1/Spi1 antibody - ab66059 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Ab66059 was coated on to an ELISA plate at a concentration of 0.01 mg/ml. Afer blocking, the immunogen (recombinant tagged fragment) was added to the wells at a range of concentrations (0.01-1000 ng/ml). A tag specific secondary antibody was added for detection at a concentration of 1 µg/ml.

Anti-PU.1/Spi1 antibody - ab77436 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PU.1/Spi1 antibody (ab77436) at 0.03 µg/ml + DAUDI lysate at 35 µgdeveloped using the ECL technique

Anti-PU.1/Spi1 antibody - ab111002 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab111002, at 3.75µg/ml, staining PU.1/Spi1 in formalin-fixed, paraffin-embedded Human Colon lymphoid aggregate by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PU.1/Spi1 antibody [EPR3158Y] - ab76543 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3158Y
All lanes : Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/10000 dilutionLane 1 : Daudi cell lysatesLane 2 : U937 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PU.1/Spi1 antibody [EPR3159Y] - ab76542 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3159Y
All lanes : Anti-PU.1/Spi1 antibody [EPR3159Y] (ab76542) at 1/1000 dilutionLane 1 : U937 cell lysatesLane 2 : TF-1 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PU.1/Spi1 antibody [G148-74] - ab48587 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone G148-74

Anti-PU.1/Spi1 antibody [SP238] - ab183327 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP238
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PU.1/Spi1 with ab183327 at 1/100.

Anti-PU.1/Spi1 antibody - N-terminal - ab135136 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-PU.1/Spi1 antibody - N-terminal (ab135136) at 1/500 dilution + THP1 total cell extract

Anti-SPI1 / PU.1 Antibody (N-Terminus) - LS-C38665 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SPI1 / PU.1 Antibody (N-Terminus) - LS-C46838 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SPI1 / PU.1 Antibody (aa61-110) - LS-C30679 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lymph Node Lysate

Anti-SPI1 / PU.1 Antibody (aa104-153) - LS-C30035 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal Thymus Lysate
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