Anti-PUMA antibody
| Name | Anti-PUMA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9643 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: PLPRGHRAPEMEPN , corresponding to C terminal amino acids 180-193 of Human PUMA alpha |
| Blocking Peptide | Human PUMA peptide |
| Description | Rabbit Polyclonal |
| Gene | BBC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with anti-PUMA at 2 µg /ml.
Ab9643 staining human ovarian carcinoma tissue. Staining is localized to mitochondria.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab9643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9643, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Preeclampsia is associated with alterations in the p53-pathway in villous - Preeclampsia is associated with alterations in the p53-pathway in villous
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A novel binuclear palladacycle complex inhibits melanoma growth in vitro and in - A novel binuclear palladacycle complex inhibits melanoma growth in vitro and in
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TP53 suppression promotes erythropoiesis in del(5q) MDS, suggesting a targeted - TP53 suppression promotes erythropoiesis in del(5q) MDS, suggesting a targeted
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Retinoblastoma protein induction by HIV viremia or CCR5 in monocytes exposed to - Retinoblastoma protein induction by HIV viremia or CCR5 in monocytes exposed to
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p53 hypersensitivity is the predominant mechanism of the unique responsiveness of - p53 hypersensitivity is the predominant mechanism of the unique responsiveness of
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Uncoupling p53 functions in radiation-induced intestinal damage via PUMA and p21. - Uncoupling p53 functions in radiation-induced intestinal damage via PUMA and p21.
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RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at - RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at
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