BBC3

Gene Symbol BBC3
Entrez Gene 27113
Alt Symbol JFY-1, JFY1, PUMA
Species Human
Gene Type protein-coding
Description BCL2 binding component 3
Other Description bcl-2-binding component 3|p53 up-regulated modulator of apoptosis
Swissprots B9EGI3 Q9BXH1 Q96PG8 O00171 Q96PG9
Accessions EAW57465 EAW57466 EAW57467 Q96PG8 Q9BXH1 AA887822 AF332558 AAK31316 AF354654 AAK39542 AF354655 AAK39543 AF354656 AAK39544 AK124984 AK298837 BAG60965 BC136481 AAI36482 BG258126 U82987 AAB51243 XM_006723141 XP_006723204 XM_011526722 XP_011525024 XM_011526723 XP_011525025 NM_001127240 NP_001120712 NM_001127241 NP_001120713 NM_001127242 NP_001120714 NM_014417 NP_055232
Function Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. {ECO:0000269|PubMed:11463391, ECO:0000269|PubMed:23340338}.
Subcellular Location Mitochondrion {ECO:0000269|PubMed:11463391, ECO:0000269|PubMed:11463392, ECO:0000269|PubMed:11572983}. Note=Localized to the mitochondria in order to induce cytochrome c release.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:11463391}.
Top Pathways p53 signaling pathway, Huntington's disease, Measles, Hippo signaling pathway

Puma Antibody - 4976 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cells using Puma Antibody.

Puma (D30C10) Rabbit mAb - 12450 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D30C10
Western blot analysis of extracts from A549 cells, untreated (-) or treated with Doxorubicin #5927 (500 nM, overnight; +), using Puma (D30C10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

PUMAα (N-20) - sc-20534 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PUMAα (N-19) - sc-19187 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PUMAα (N-19): sc-19187. Western blot analysis of PUMAα expression in K-562 (A), Jurkat (B), MOLT-4 (C) and U-937 (D) whole cell lysates.

PUMAα/β (G-16) - sc-19188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PUMAα/β (H-136) - sc-28226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PUMAα/β (H-136): sc-28226. Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic and perinuclear staining.

PUMAα/β (D-20) - sc-20536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PUMAα/β (C-14) - sc-19189 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PUMAδ (D-13) - sc-19191 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PUMAδ (D-13): sc-19191. Immunofluorescence staining of methanol-fixed Jurkat cells showing cytoplasmic localization.

PUMAβ/δ (P-13) - sc-19190 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PUMA alpha antibody [2A9G5] - ab181005 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2A9G5
Lane 1 : Anti-PUMA alpha antibody [2A9G5] (ab181005) at 2.5 µg/mlLane 2 : Anti-PUMA alpha antibody [2A9G5] (ab181005) at 5 µg/mlLane 1 : K562 cell lysateLane 2 : K562 cell lysateLysates/proteins at 15 µg per lane.

Anti-PUMA alpha antibody - C-terminal - ab182788 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PUMA alpha antibody - C-terminal (ab182788) at 1/540 dilutionLane 1 : NIH3T3 cell lysateLane 2 : A549 cell lysateLane 3 : Hela cell lysateLysates/proteins at 30 µg per lane.SecondaryGoat anti Rabbit IgG at 1/10000 dilution

Anti-PUMA antibody - ab9643 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with anti-PUMA at 2 µg /ml.

Anti-PUMA antibody - ab9645 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PUMA antibody (ab9645) at 2 µg/ml + human K562 cell lysatePerformed under reducing conditions.

Anti-PUMA antibody - ab54288 from Abcam

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PUMA antibody (ab54288) at 1/1000 dilutionLane 1 : Cell lysates prepared form human K562 cellsLane 2 : Cell lysates prepared from mouse 3T3 cells

Anti-PUMA antibody - ab27669 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PUMA antibody - ab26802 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab26802 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26802, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PUMA antibody - ab109548 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-PUMA antibody (ab109548) at 0.1 µg/ml + Peripheral Blood Lymphocytes lysate at 35 µg

Anti-PUMA antibody [EP512Y] - ab33906 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP512Y
Anti-PUMA antibody [EP512Y] (ab33906) at 1/5000 dilution + K562 cell lysate.

Anti-PUMA antibody - C-terminal - ab189123 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling PUMA with ab189123 at 10 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-BBC3 / PUMA Antibody (C-Terminus, DY488) - LS-C142275 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PUMA Antibody (C-Terminus, HRP) - LS-C142276 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BBC3 / PUMA Antibody (C-Terminus, DY550) - LS-C142277 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BBC3 / PUMA Antibody (aa21-70) - LS-C117030 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
BBC3 / PUMA antibody LS-C117030 Western blot of Placenta lysate.

Anti-BBC3 / PUMA Antibody (C-Terminus, Sepharose) - LS-C171987 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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