Anti-PUMA antibody [EP512Y]
| Name | Anti-PUMA antibody [EP512Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab33906 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP512Y |
| Applications | FC WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to residues in the C-terminal of Human PUMA |
| Blocking Peptide | PUMA peptide |
| Description | Rabbit Monoclonal |
| Gene | BBC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-PUMA antibody [EP512Y] (ab33906) at 1/5000 dilution + K562 cell lysate.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/18718_ab33906_1.jpg)
Anti-PUMA antibody [EP512Y] (ab33906) at 1/5000 dilution + K562 cell lysate.
ab33906 at a 1:100 dilution staining PUMA in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
ab33906 at a 1:100 dilution staining PUMA in human ovary carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
ab33906 at a 1:250 dilution staining PUMA in Hela cells using Immunocytochemistry/Immunofluorescence.
Overlay histogram showing K562 cells stained with ab33906 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33906, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in K562 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
Product References
Methotrexate-mediated inhibition of nuclear factor kappaB activation by distinct - Methotrexate-mediated inhibition of nuclear factor kappaB activation by distinct
Spurlock CF 3rd, Gass HM 4th, Bryant CJ, Wells BC, Olsen NJ, Aune TM. Rheumatology (Oxford). 2015 Jan;54(1):178-87.
ASPP1/2 regulate p53-dependent death of retinal ganglion cells through PUMA and - ASPP1/2 regulate p53-dependent death of retinal ganglion cells through PUMA and
Wilson AM, Morquette B, Abdouh M, Unsain N, Barker PA, Feinstein E, Bernier G, Di Polo A. J Neurosci. 2013 Jan 30;33(5):2205-16.
Transcription factor Sox4 is required for PUMA-mediated apoptosis induced by - Transcription factor Sox4 is required for PUMA-mediated apoptosis induced by
Jang SM, Kang EJ, Kim JW, Kim CH, An JH, Choi KH. Biochem Biophys Res Commun. 2013 Aug 23;438(2):445-51. doi:
Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute - Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute
Yang CF, Peng LX, Huang TJ, Yang GD, Chu QQ, Liang YY, Cao X, Xie P, Zheng LS, Huang HB, Cai MD, Huang JL, Liu RY, Zhu ZY, Qian CN, Huang BJ. Cancer Lett. 2014 Mar 28;344(2):260-71.
Apoptosis induction by SAHA in cutaneous T-cell lymphoma cells is related to - Apoptosis induction by SAHA in cutaneous T-cell lymphoma cells is related to
Al-Yacoub N, Fecker LF, Mobs M, Plotz M, Braun FK, Sterry W, Eberle J. J Invest Dermatol. 2012 Sep;132(9):2263-74.
Mutual regulation of Bcl-2 proteins independent of the BH3 domain as shown by the - Mutual regulation of Bcl-2 proteins independent of the BH3 domain as shown by the
Plotz M, Hossini AM, Gillissen B, Daniel PT, Stockfleth E, Eberle J. PLoS One. 2012;7(4):e34549.
Omeprazole inhibits proliferation and modulates autophagy in pancreatic cancer - Omeprazole inhibits proliferation and modulates autophagy in pancreatic cancer
Udelnow A, Kreyes A, Ellinger S, Landfester K, Walther P, Klapperstueck T, Wohlrab J, Henne-Bruns D, Knippschild U, Wurl P. PLoS One. 2011;6(5):e20143.
Increased sensitivity to apoptosis induced by methotrexate is mediated by JNK. - Increased sensitivity to apoptosis induced by methotrexate is mediated by JNK.
Spurlock CF 3rd, Aune ZT, Tossberg JT, Collins PL, Aune JP, Huston JW 3rd, Crooke PS, Olsen NJ, Aune TM. Arthritis Rheum. 2011 Sep;63(9):2606-16.
Identifying catalytic residues in CPAF, a Chlamydia-secreted protease. - Identifying catalytic residues in CPAF, a Chlamydia-secreted protease.
Chen D, Chai J, Hart PJ, Zhong G. Arch Biochem Biophys. 2009 May 1;485(1):16-23.
An Epstein-Barr virus-encoded microRNA targets PUMA to promote host cell - An Epstein-Barr virus-encoded microRNA targets PUMA to promote host cell
Choy EY, Siu KL, Kok KH, Lung RW, Tsang CM, To KF, Kwong DL, Tsao SW, Jin DY. J Exp Med. 2008 Oct 27;205(11):2551-60.