Anti-Pumilio 1 antibody [EPR3795]

• Supplier: Abcam
• Catalog: ab92545
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3795
• Applications: ICC/IF ICC/IF WB IP FC IHC-P
• Species Reactivities: Mouse, Human
• Antigen: A synthetic peptide corresponding to residues in Human Pumilio 1
• Description: Rabbit Monoclonal
• Gene: PUM1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using ab92545 at a 1/100 dilution.

Immunofluorescent staining of Pumilio 1 in HeLa cells using ab92545 at a 1/100 dilution.

Overlay histogram showing HEK293 cells stained with ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.