Anti-Pumilio 2 antibody
| Name | Anti-Pumilio 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10361 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rabbit, Horse, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan, Elephant |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human Pumilio homolog 2 encoded within exon 4 (LocusLink ID 23369) |
| Description | Rabbit Polyclonal |
| Gene | PUM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab10361 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10361 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Translational regulation of acetylcholinesterase by the RNA-binding protein - Translational regulation of acetylcholinesterase by the RNA-binding protein
Marrero E, Rossi SG, Darr A, Tsoulfas P, Rotundo RL. J Biol Chem. 2011 Oct 21;286(42):36492-9.
Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. - Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function.
Vessey JP, Schoderboeck L, Gingl E, Luzi E, Riefler J, Di Leva F, Karra D, Thomas S, Kiebler MA, Macchi P. Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3222-7. doi:
Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry - Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry
Barrios F, Filipponi D, Pellegrini M, Paronetto MP, Di Siena S, Geremia R, Rossi P, De Felici M, Jannini EA, Dolci S. J Cell Sci. 2010 Mar 15;123(Pt 6):871-80.
Potential role of Nanos3 in maintaining the undifferentiated spermatogonia - Potential role of Nanos3 in maintaining the undifferentiated spermatogonia
Lolicato F, Marino R, Paronetto MP, Pellegrini M, Dolci S, Geremia R, Grimaldi P. Dev Biol. 2008 Jan 15;313(2):725-38. Epub 2007 Nov 19.