PUM2

Gene Symbol PUM2
Entrez Gene 23369
Alt Symbol PUMH2, PUML2
Species Human
Gene Type protein-coding
Description pumilio RNA-binding family member 2
Other Description pumilio homolog 2|pumilio-2
Swissprots B3KSL0 Q9HAN2 Q8WY43 Q53TV7 B4E2B6 O00234 D6W527 Q8TB72
Accessions AAY15026 EAX00821 EAX00822 EAX00823 EAX00824 Q8TB72 AF272350 AAL36981 AF315591 AAG31806 AK093847 BAG52772 AK304198 BAG65078 BC024218 AAH24218 BC112046 AAI12047 BC112048 AAI12049 BC143550 AAI43551 BC143552 BG717417 BX641165 D87078 BAA19665 XM_005262607 XP_005262664 XM_005262609 XP_005262666 XM_005262610 XP_005262667 XM_006711973 XP_006712036 XM_006711974 XP_006712037 XM_006711975 XP_006712038 XM_011532719 XP_011531021 XM_011532720 XP_011531022 XM_011532721 XP_011531023 XM_011532722 XP_011531024 XM_011532723 XP_011531025 XM_011532724 XP_011531026 XM_011532725 XP_011531027 NM_001282752 NP_001269681 NM_001282790 NP_001269719 NM_001282791 NP_001269720 NM_015317 NP_056132
Function Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. {ECO:0000269|PubMed:21397187, ECO:0000269|PubMed:22955276}.
Subcellular Location Cytoplasm {ECO:0000305|PubMed:19168546}. Cytoplasmic granule {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:19168546}. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells. {ECO:0000250}.
Tissue Specificity Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. {ECO:0000269|PubMed:12459267, ECO:0000269|PubMed:12511597, ECO:0000269|PubMed:19168546}.

Pumilio 2 Antibody - 12323 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A172 and NTERA-2 cl.D1 cells using Pumilio 2 Antibody.

Pumilio 2 (A-10) - sc-398632 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pumilio 2 (A-10): sc-398632. Western blot analysis of Pumilio 2 expression in non-transfected: sc-117752 (A) and mouse Pumilio 2 transfected: sc-122843 (B) 293T whole cell lysates.

Pumilio 2 (C-8) - sc-514108 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pumilio 2 (C-8): sc-514108. Western blot analysis of Pumilio 2 expression in ES-2 (A), NTERA-2 cl.D1 (B), HeLa (C), RT-4 (D), U-251-MG (E) and Hs 181 Tes (F) whole cell lysates.

Pumilio 2 (K-14) - sc-31535 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pumilio 2 (K-14): sc-31535. Western blot analysis of Pumilio 2 expression in non-transfected 293T: sc-117752 (A), mouse Pumilio 2 transfected 293T: sc-122843 (B), IMR-32 (C) and Jurkat (D) whole cell lysates.

Pumilio 2 (D-17) - sc-31537 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pumilio 2 (D-16) - sc-31536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pumilio 2 (P-16) - sc-31538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pumilio 2 (P-21) - sc-133936 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Pumilio 2 (P-21): sc-133936. Western blot analysis of human Pumilio 2 transfected 293T whole cell lysate.

Anti-Pumilio 2 antibody - ab10361 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Pumilio 2 antibody - ab190104 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Pumilio 2 with ab190104 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-Pumilio 2 antibody - ab32894 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-Pumilio 2 antibody (ab32894) at 1 µg/mlLane 1 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - without blocking with the immunizing peptideLane 2 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - blocking with the immunizing peptideLysates/proteins at 35 µg per lane.

Anti-Pumilio 2 antibody - ab76694 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Pumilio 2 antibody (ab76694) at 1/50 dilutionLane 1 : 293 cell lysates, nontransfected Lane 2 : 293 cell lysates transiently transfected with the Pumilio 2 geneLysates/proteins at 2 µg per lane.

Anti-Pumilio 2 antibody - ab57049 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57049 Pumilio 2 antibody at 1ug/ml.

Anti-Pumilio 2 antibody [EPR3813] - ab92390 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3813
All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PUM2 Antibody (aa100-150) - LS-C287126 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Anti-PUM2 Antibody (N-Terminus) IHC-plus™ - LS-B9061 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-PUM2 Antibody (Ser182) IHC-plus™ - LS-B9123 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-PUM2 Antibody (aa100-150) - LS-C93492 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PUM2 Antibody - LS-C131749 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PUM2 Antibody (clone 3E7) - LS-C139522 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E7

Anti-PUM2 Antibody (aa101-150) - LS-C40322 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-PUM2 Antibody (N-Terminus, APC) - LS-C281702 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PUM2 Antibody (N-Terminus, AP) - LS-C281704 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PUM2 Antibody (N-Terminus, Biotin) - LS-C281703 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PUM2 Antibody (N-Terminus, FITC) - LS-C281705 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more