All lanes : Anti-Pumilio 2 antibody (ab32894) at 1 µg/mlLane 1 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - without blocking with the immunizing peptideLane 2 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - blocking with the immunizing peptideLysates/proteins at 35 µg per lane.
Lane 1 : Anti-Pumilio 2 antibody (ab32894) at 0.5 µg/mlLane 2 : Anti-Pumilio 2 antibody (ab32894) at 1 mg/mlLane 3 : anti- DYKDDDDK Tag at 1/3000 dilutionLane 1 : HEK293 lysate overexpressing Human PUM2 with DYKDDDDK tag at 10 µgLane 2 : Mock Tansfected HEK293Lane 3 : HEK293 lysate overexpressing Human PUM2 with DYKDDDDK tag at 10 µg