All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilutionLane 1 : HeLa whole cell lysate- 18µgLane 2 : HeLa whole cell lysate- 36µgLane 3 : RPE-1 whole cell lysate- 18µgLane 4 : RPE-1 whole cell lysate- 36µgSecondaryLICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilutionPerformed under reducing conditions.
Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg
All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilutionLane 1 : PYCR2 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 25 µg per lane.