Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8]

• Supplier: Abcam
• Catalog: ab110331
• Prices: $382.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 17A5E2H8
• Applications: IHC-P WB
• Species Reactivities: Mouse, Rat, Bovine, Human
• Antigen: Porcine Pyruvate Dehydrogenase E1 beta subunit protein
• Description: Mouse Monoclonal
• Gene: PDHB
• Conjugate: Unconjugated

Product images

IHC image of Pyruvate Dehydrogenase E1 beta subunit staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8] (ab110331) at 1 µg/mlLane 1 : Isolated mitochondria from Human heart at 10 µg/mlLane 2 : Isolated mitochondria from Bovine heart at 4 µg/mlLane 3 : Isolated mitochondria from Rat heart at 10 µg/mlLane 4 : Isolated mitochondria from Mouse heart at 10 µg/mlLane 5 : HepG2 cell lysate at 20 µg/ml

Product References

Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic - Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic

Halim ND, Mcfate T, Mohyeldin A, Okagaki P, Korotchkina LG, Patel MS, Jeoung NH, Harris RA, Schell MJ, Verma A. Glia. 2010 Aug;58(10):1168-76.

A combined therapeutic approach for pyruvate dehydrogenase deficiency using - A combined therapeutic approach for pyruvate dehydrogenase deficiency using

Han Z, Berendzen K, Zhong L, Surolia I, Chouthai N, Zhao W, Maina N, Srivastava A, Stacpoole PW. Mol Genet Metab. 2008 Apr;93(4):381-7.

Identification of proteins undergoing tyrosine phosphorylation during mouse sperm - Identification of proteins undergoing tyrosine phosphorylation during mouse sperm

Arcelay E, Salicioni AM, Wertheimer E, Visconti PE. Int J Dev Biol. 2008;52(5-6):463-72.