PDHB

Gene Symbol PDHB
Entrez Gene 5162
Alt Symbol PDHBD, PDHE1-B, PHE1B
Species Human
Gene Type protein-coding
Description pyruvate dehydrogenase (lipoamide) beta
Other Description pyruvate dehydrogenase E1 component subunit beta, mitochondrial|pyruvate dehydrogenase, E1 beta polypeptide
Swissprots B2R7L0 Q9UFK3 Q9BQ27 Q6FH45 B4DDD7 P11177
Accessions BAA14123 EAW65371 EAW65372 EAW65373 P11177 AB209241 BAD92478 AK225569 AK225621 AK293153 BAG56698 AK311514 AK313022 BAG35857 AL117618 CAB56017 AL706543 AM392999 CAL37877 AM393278 CAL38156 BC000439 AAH00439 BC001924 AAH01924 BC004115 BM971427 CR541911 CAG46709 DA965753 DB039833 DQ891366 ABM82292 DQ894546 ABM85472 J03576 AAA88097 M19123 AAA60052 M19124 M34055 AAA60233 M34056 AAA60054 M34479 AAA36428 M54788 AAA60053 X57778 CAA40924 XM_011533828 XP_011532130 NM_000925 NP_000916 NM_001173468 NP_001166939 NR_033384
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. {ECO:0000269|PubMed:17474719, ECO:0000269|PubMed:19081061}.
Subcellular Location Mitochondrion matrix.
Top Pathways Glycolysis / Gluconeogenesis, Citrate cycle (TCA cycle), Central carbon metabolism in cancer, Glucagon signaling pathway, Pyruvate metabolism

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody - ab154541 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab154541) at 1/1000 dilutionLane 1 : A549 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : HCT116 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody - ab168446 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab168446) at 1 µg/mlLane 1 : Transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat Anti-mouse HRP at 1/2500 dilution

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8] - ab110331 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 17A5E2H8
IHC image of Pyruvate Dehydrogenase E1 beta subunit staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11096(B)] - ab155953 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11096(B)
ab155953 showing +ve staining in Human thyroid gland carcinoma.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11097(B)] - ab155996 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11097(B)
ab155996 showing +ve staining in Human normal pancreas.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody - ab55574 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Pyruvate Dehydrogenase E1 beta subunit antibody (ab55574) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody - ab126203 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab126203 stained HepG2 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126203, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody - ab84588 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab84588) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PDHB Antibody (aa65-336, Biotin) - LS-C298106 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDHB Antibody (aa31-341, Biotin) - LS-C298107 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDHB Antibody (aa31-341, FITC) - LS-C302599 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDHB Antibody (aa65-336, FITC) - LS-C302628 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PDHB Antibody (aa250-360, Azide-free) - LS-C328412 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-PDHB Antibody (aa72-121) - LS-C102291 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PDHB antibody LS-C102291 Western blot of Fetal Heart lysate.

Anti-PDHB Antibody (aa31-341) - LS-C296161 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant PDHB.

Anti-PDHB Antibody (aa65-336) - LS-C296162 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant PDHB.

Anti-PDHB Antibody (clone 2B2) - LS-C133369 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B2
Immunoperoxidase of monoclonal antibody to PDHB on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]

Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) - H00005162-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B2
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in HeLa (Cat # L013V1).

Pyruvate Dehydrogenase E1 beta subunit Antibody - NBP2-20024 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20024] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 10% SDS PAGE gel, diluted at 1:10000.

Pyruvate Dehydrogenase E1 beta subunit Antibody - NBP2-38327 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-38327] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Pyruvate Dehydrogenase E1 beta subunit Antibody - NBP2-20025 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP2-20025] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Pyruvate Dehydrogenase E1 beta subunit Antibody - H00005162-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Pyruvate Dehydrogenase E1 beta subunit Antibody - H00005162-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [H00005162-B01] - PDHB MaxPab polyclonal antibody. Western Blot analysis of PDHB expression in rat brain.

Pyruvate Dehydrogenase E1 beta subunit Antibody - NBP1-87421 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Pyruvate Dehydrogenase E1 beta subunit Antibody - H00005162-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [H00005162-D01P] - Analysis of PDHB expression in transfected 293T cell line by PDHB polyclonal antibody.Lane 1: PDHB transfected lysate(39.20 KDa).Lane 2: Non-transfected lysate.
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