All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody [EPR12200] (ab177461) at 1/1000 dilutionLane 1 : Immunoprecipitation pellet from 293T cells lysate at 10 µgLane 2 : 1X PBS (negative control)SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Pyruvate dehydrogenase E1-alpha subunit with ab177461 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Pyruvate dehydrogenase E1-alpha subunit with ab177461 at 1/50 dilution.
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody [EPR12200] (ab177461) at 1/1000 dilutionLane 1 : 293T cell lysates untreatedLane 2 : 293T cell lysates, membrane treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique